Sekuen Internal Transcribed Spacer (Its) Dna Ribosomal Oncobasidium Theubromae Dan Jamur Sekerabat Pembanding
Agustin Sri Mulyatni
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Deskripsi Buku
Abstract INTERNAL TRANSCRIBED SPACER ITS SEQUENCES OF RIBOSOMAL DNA ONCOBASIDIUM THEOBROMAE AND OTHER RELATED FUNGI AS COMPARISON AGUSTIN SRI MULYATNI 02 156325 PN 09316 The purposes of this research was to sequence ITS region from ribosomal DNA of Oncobasidium theobromae and compare its sequences to the isolates from Vietnam and Malaysia and also to identify the other related fungi species based on the homology of ITS region Oncobasidium theobromae was isolated from three cocoa plantations in Indonesia that were Jember Kendari and Makassar DNA was isolated from the fungal mycelia and then amplified using ITS 4 and ITS 5 primers resulted in DNA fragments of 600 bp and 700 bp for the isolate of Jember 600 bp for the isolate of Kendari and 700 bp for the isolate of Makassar All the fragments were successfully sequenced except for 600 bp fragment of the isolate of Jember The homology analysis utilized BLAST prooved that ITS sequences Oncobasidium theobromae from Jember was homolog with Rhizoctonia sp whereas isolate of Kendari was homolog with Botryosphaeria sp and isolate of Makassar was homolog with Mycorrhizal basidiomycetes The sequences were then compared to the sequences of Oncobasidium theobromae from Vietnam and Malaysia Phylogenetic analyses using Clustal W program indicate that Oncobasidium theobrome from Indonesia which is represented by isolates from Jember show higher degree of similarity to isolates from Vietnam On the contrary isolates from Indonesia show lower degree of similarity to isolates from Malaysia Key words homology analysis ITS Oncobasidium theobromae rDNA VSDAbstract INTERNAL TRANSCRIBED SPACER ITS SEQUENCES OF RIBOSOMAL DNA ONCOBASIDIUM THEOBROMAE AND OTHER RELATED FUNGI AS COMPARISON AGUSTIN SRI MULYATNI 02 156325 PN 09316 The purposes of this research was to sequence ITS region from ribosomal DNA of Oncobasidium theobromae and compare its sequences to the isolates from Vietnam and Malaysia ...and also to identify the other related fungi species based on the homology of ITS region Oncobasidium theobromae was isolated from three cocoa plantations in Indonesia that were Jember Kendari and Makassar DNA was isolated from the fungal mycelia and then amplified using ITS 4 and ITS 5 primers resulted in DNA fragments of 600 bp and 700 bp for the isolate of Jember 600 bp for the isolate of Kendari and 700 bp for the isolate of Makassar All the fragments were successfully sequenced except for 600 bp fragment of the isolate of Jember The homology analysis utilized BLAST prooved that ITS sequences Oncobasidium theobromae from Jember was homolog with Rhizoctonia sp whereas isolate of Kendari was homolog with Botryosphaeria sp and isolate of Makassar was homolog with Mycorrhizal basidiomycetes The sequences were then compared to the sequences of Oncobasidium theobromae from Vietnam and Malaysia Phylogenetic analyses using Clustal W program indicate that Oncobasidium theobrome from Indonesia which is represented by isolates from Jember show higher degree of similarity to isolates from Vietnam On the contrary isolates from Indonesia show lower degree of similarity to isolates from Malaysia Key words homology analysis ITS Oncobasidium theobromae rDNA VSD
