Optimazation Of Dna Extraction Methods From Fusarium Oxysporum F.Sp Cubense For Pcr Analysis
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IV RESULTS AND DISCUSSION DNA sample was extracted by three different DNA extraction methods CTAB method resulted on thicker DNA bands than the other methods figure 4 1 It meant that this method was able to obtain more DNA samples than the other CTAB cetyltrimethylammonium bromide is a kind of detergent which can degrade cell wall denaturizes proteins separate carbohydrates safely Kaidah dan Suprapto 2003 and dissolve DNA samples Purwantara 2001 Weiland 2005 reported that CTAB method was considered superior at removing unwanted carbohydrate from DNA preparations Figure 4 1 DNA extraction using CTAB method Lane 1 isolate Bntl 2 isolate Bnt2 3 isolate Wsb3 4 isolate Btu3 5 isolate Kjgl 6 isolate Lmp3 The others components such as EDTA ethylenediamine tetra acetate can serve as chelating agent binding magnesium a cofactor in the enzymes activity such as endonuclease degrading DNA samples Purwantara 2001 and 2 Mercapthoethanol can protect DNA samples from compound quinines disulphide peroxides poliphenoloxidases and proteins Milligan 1992 Alkaline Lysis method and SDS method showed thinner DNA bands It indicated that these methods could merely extract less DNA samples figure 4 2A and figure 4 2B The extraction buffer of Alkaline Lysis method does not contain NaCl as a salt precipitating DNA while the extraction buffer of SDS contains NaCl in lower concentration 0 5 M than the extraction buffer of CTAB method 1 4 M Therefore these methods require addition of salt the former needs 3 M CH3COONa and the latter needs 5 M CH3COOK Purwantara 2001 reported that in general NaCl in higher 14 fpAKUtTA i IV RESULTS AND DISCUSSION DNA sample was extracted by three different DNA extraction methods CTAB method resulted on thicker DNA bands than the other methods figure 4 1 It meant that this method was able to obtain more DNA samples than the other CTAB cetyltrimethylammonium bromide is a kind of detergent ...which can degrade cell wall denaturizes proteins separate carbohydrates safely Kaidah dan Suprapto 2003 and dissolve DNA samples Purwantara 2001 Weiland 2005 reported that CTAB method was considered superior at removing unwanted carbohydrate from DNA preparations Figure 4 1 DNA extraction using CTAB method Lane 1 isolate Bntl 2 isolate Bnt2 3 isolate Wsb3 4 isolate Btu3 5 isolate Kjgl 6 isolate Lmp3 The others components such as EDTA ethylenediamine tetra acetate can serve as chelating agent binding magnesium a cofactor in the enzymes activity such as endonuclease degrading DNA samples Purwantara 2001 and 2 Mercapthoethanol can protect DNA samples from compound quinines disulphide peroxides poliphenoloxidases and proteins Milligan 1992 Alkaline Lysis method and SDS method showed thinner DNA bands It indicated that these methods could merely extract less DNA samples figure 4 2A and figure 4 2B The extraction buffer of Alkaline Lysis method does not contain NaCl as a salt precipitating DNA while the extraction buffer of SDS contains NaCl in lower concentration 0 5 M than the extraction buffer of CTAB method 1 4 M Therefore these methods require addition of salt the former needs 3 M CH3COONa and the latter needs 5 M CH3COOK Purwantara 2001 reported that in general NaCl in higher 14 fpAKUtTA i
